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. 2014 Nov 25;33(24):2997–3011. doi: 10.15252/embj.201490230

Figure 5. A C-terminal region of βPIX is required for binding to YAP and LATS.

Figure 5

  • A A schematic depicting the different βPIX cDNA constructs used for mapping interactions is shown. Positive or negative interactions with YAP and LATS1 are indicated on the right.
  • B, C HEK293T cells were co-transfected with wild-type or mutant constructs of Flag-βPIX along with HA-YAP (B) or HA-LATS1 (C). Cell lysates were subjected to anti-Flag IP, and the presence of YAP or LATS1 was determined by anti-HA immunoblotting. A dashed line on blots indicates removal of a sample lane.
  • D, E Quantitation of βPIX interaction mapping from replicate experiments YAP (D), n = 3, and LATS1 (E), n = 2, is plotted.

Source data are available online for this figure.