Table 3.
Similarity of sequences of 16S rDNA DGGE bands determined by BLAST nucleotide search
| DGGE bands | Closest database match of cultured taxa (accession) | Similarity (%) | Taxonomic position (lower systematic taxa) | Closest database match (accession) | Similarity (%) |
|---|---|---|---|---|---|
| 0 | Acidisphaera sp. (AB669479.1) | 88 | Alphaproteobacteria (Acetobacteriaceae) | Uncultured bacterium clone FH-2-29 16S ribosomal RNA gene, partial sequence (GQ162031.1) | 91 |
| 1 | Pseudomonas syringae (AJ534944.1) | 76 | Gammaproteobacteria (Pseudomonadales) | Uncultured Desulfomicrobium sp. clone GE7GXPU01B1Z87 16S ribosomal RNA gene, partial sequence (HM508007.1) | 76 |
| 2 | Sphingopyxis sp. (HM224464.1) | 92 | Alphaproteobacteria (Sphingomonadales) | Uncultured Novosphingobium sp. clone fjc-77 16S ribosomal RNA gene, partial sequence (JQ278820.1) | 94 |
| 2b | Sphingopyxis sp. (HM224464.1) | 94 | Alphaproteobacteria (Sphingomonadales) | Uncultured Novosphingobium sp. clone fjc-77 16S ribosomal RNA gene, partial sequence (JQ278820.1) | 96 |
| 3 | Bacterium enrichment culture clone B30 (2011) 16S ribosomal RNA gene, partial sequence (JF830203.1) | 96 | Deltaproteobacteria | Uncultured Desulfuromonadales bacterium partial 16S rRNA gene, clone AMBD2 (AM935739.1) | 99 |
| 3b | Woodsholea maritima (FM886859.2) | 97 | Alphaproteobacteria (Rhodobacteriaceae) | Uncultured alphaproteobacterium clone W-LFP137 16S ribosomal RNA gene, partial sequence (JF516154.1) | 99 |
| 4 | Methylotenera mobilis (CP001672.1) | 99 | Betaproteobacteria (Methylophilaceae) | Uncultured Methylophilaceae bacterium clone LYH1-18 16S ribosomal RNA gene, partial sequence (JQ994354.1) | 99 |
| 4b | Methylophilus sp. (EU375653.1) | 93 | Betaproteobacteria (Methylophilaceae) | Uncultured bacterium clone P_21 16S ribosomal RNA gene, partial sequence (JQ810586.1) | 93 |
| 5 | Acidobacterium sp. (FN689719.1) | 93 | Deltaproteobacteria (Acidobacteria) | Uncultured bacterium clone EMIRGE_OTU_s2b2b_10774 16S ribosomal RNA gene, partial sequence (JX222537.1) | 96 |
| 5b | Acidobacterium sp. (FN689719.1) | 96 | Deltaproteobacteria (Acidobacteria) | Uncultured bacterium clone EMIRGE_OTU_s2b2b_10774 16S ribosomal RNA gene, partial sequence (JX222537.1) | 96 |
| 6 | Rhodovulum sp. (EU642859.1) | 93 | Alphaproteobacteria (Rhodobacteriaceae) | Uncultured bacterium clone a-19 16S ribosomal RNA gene, partial sequence (JX040364.1) | 94 |
| 6b | Geothrix fermentans (NR036779.1) | 94 | Deltaproteobacteria (Acidobacteria) | Uncultured bacterium clone EMIRGE_OTU_s2b2b_3195 16S ribosomal RNA gene, partial sequence (JX222379.1) | 94 |
| 7 | Uncultured bacterium clone a-132 16S ribosomal RNA gene, partial sequence (JX040403.1) | 92 | |||
| 7b | Uncultured bacterium clone a-132 16S ribosomal RNA gene, partial sequence (JX040403.1) | 98 | |||
| 8b | Rhodobacter blasticus (NR043735.1) | 98 | Alphaproteobacteria (Rhodobacteriaceae) | uncultured bacterium partial 16S rRNA gene, clone K15.94 AW (HE576390.1) | 99 |
| 9 | Lacibacter cauensis (AB682227.1) | 97 | Bacteroidetes | Uncultured Bacteroidetes bacterium clone VSL5W1u13 16S ribosomal RNA gene, partial sequence (EU633854.1) | 99 |
| 9b | Sporocytophaga sp. (DQ186971.1) | 87 | Bacteroidetes | Uncultured Bacteroidetes bacterium clone cher4_1B_19 small subunit ribosomal RNA gene, partial sequence (JN020226.1) | 88 |
| 10 | Adhaeribacter aerolatus (HM156145.1) | 87 | Bacteroidetes | Adhaeribacter aerolatus (HM156145.1) | 87 |
| 10b | Sporocytophaga sp. (DQ186971.1) | 88 | Bacteroidetes | Uncultured bacterium clone B116 16S ribosomal RNA gene, partial sequence (JF429098.1) | 89 |