Fig. 7.
Drp1K38A mutant overexpression improves axonal survival following capsaicin treatment in vitro. a 1–c 2 Confocal images of control (control; a 1, a 2), wild-type Drp1-transfected (Drp1WT; b 1, b 2) and mutated Drp1 (Drp1K38A; c 1, c 2)-transfected dorsal root ganglia (DRG) cultures immunostained for PGP9.5 48 h after capsaicin (CAP) or DMSO treatment. Capsaicin induced significant axonal loss in control (a 1, a 2, d) and Drp1WT-transfected (b 1, b 2, d) cultures. Axonal density was rescued in Drp1K38A-transfected (c 1, c 2, d) cultures (***P < 0.0001, One-Way ANOVA). The analysis was based on 3–4 randomly chosen fields in each replicate. Dots represent number of replicates. Scale bars a 1 –c 2 100 μm