. 2014 Sep 27;34(1):47–62. doi: 10.1007/s00299-014-1686-4

Table 2.

The effect of cold treatment (3 weeks at 4 °C) on plant growth regulators content in triticale (× Triticosecale Witm.) anthers

Variable	‘recalcitrant’ DH lines		‘responsive’ DH lines
Variable	FC	CT	FC	CT
IAA (nmol g⁻¹ DW)	23.2^b	28.7^c	16.2^a	21.4^b
IBA (nmol g⁻¹ DW)	0.19^a	0.27^b	0.20^a	0.40^c
tZ (pmol g⁻¹ DW)	7.4^c	3.5^a	10.5^d	5.0^b
cZ (pmol g⁻¹ DW)	196.8^b	325.0^c	155.0^a	367.6^d
tZR (pmol g⁻¹ DW)	4.6^a	3.9^a	5.7^b	4.1^a
cZR (pmol g⁻¹ DW)	225.5^a	321.0^b	212.2^a	387.4^c
KIN (pmol g⁻¹ DW)	39.6^b	32.5^b	34.2^b	20.1^a
ABA (nmol g⁻¹ DW)	0.90^a	1.92^b	0.84^a	2.98^c
Auxs Eq/CKs Eq	49.4	42.2	39.3	27.8
Auxs Eq/ABA	26.0	15.1	19.5	7.3
CKs Eq/ABA (×10³)	526.6	357.2	497.1	263.2

Data are the means of four DH lines of triticale identified as ‘recalcitrant’ and ‘responsive’ to androgenesis induction and 3–5 biological replications for each DH line

Each Petri dish containing 100 anthers from a different spike was assumed to be one biological replication

Data marked with the same letter do not differ significantly according to the Ducan test (p ≤ 0.05)

IAA indole-3-acetic acid, IBA indolebutyric acid, tZ trans zeatin, cZ cis zeatin, tZR trans zeatin riboside, cZR cis zeatin riboside, KIN kinetin, ABA abscisic acid, Aux Eq auxin equivalent, the sum of IAA and IBA concentrations, CKs Eq cytokinins equivalent, the sum of tZ, cZ, tZR, cZT and KIN concentrations