Table 5.
The effect of auxin transport inhibitor (10 µM TIBA) and anti-auxin (5 µM PCIB) tillers treatment on endogenous level of IAA and IBA in anthers of two DH lines of triticale (× Triticosecale Wittm.) with significantly different androgenic responsiveness (‘responsive’ DH28 and ‘recalcitrant’ DH19)
| Treatment | Days of treatment | IAA (nmol g−1 DW) | IBA (nmol g−1 DW) | ||
|---|---|---|---|---|---|
| DH19 | DH28 | DH19 | DH28 | ||
| Control* | 30.5 ± 4.1 | 19.2 ± 0.8 | 0.38 ± 0.03 | 0.33 ± 0.01 | |
| TIBA | 3 | 41.1 ± 4.8 | 26.7 ± 3.1 | 0.31 ± 0.01 | 0.38 ± 0.04 |
| 21 | 14.6 ± 7.7 | 6.0 ± 0.2 | 2.1 ± 0.31 | 0.43 ± 0.02 | |
| PCIB | 3 | 14.1 ± 1.5 | 23.8 ± 2.8 | 0.96 ± 0.20 | 0.33 ± 0.04 |
| 21 | 27.7 ± 3.1 | 41.8 ± 6.0 | 0.53 ± 0.13 | 0.33 ± 0.01 | |
| DMSO | 3 | 19.1 ± 0.8 | 42.5 ± 4.0 | 1.02 ± 0.01 | 0.36 ± 0.01 |
| 21 | 46.6 ± 13.9 | 26.7 ± 8.5 | 0.40 ± 0.02 | 0.39 ± 0.01 | |
DMSO (PCIB solvent) in 0.1 % concentration was used as additional control. All compounds were supplemented to Hogland’s medium at the start of cold tillers treatment (21 days at 4 °C) or 3 days before anthers isolation
Data are the means of 3 biological replications ±SE
Sample of anthers collected from several spikes with total fresh weight of 0.3 g was assumed to be one biological replication
TIBA 2,3,5-triiodobenzoic acid, PCIB p-chlorophenoxyisobutyric acid, DMSO dimethyl sulfoxide
* Anthers excised from tillers kept during the whole cold treatment (21 days) in Hoagland’s medium