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. 2014 Dec 19;11:111. doi: 10.1186/s12977-014-0111-y

Figure 3.

Figure 3

Effect of dNTP concentration on DNA-dependent DNA polymerization activity for lentiviral RT proteins. The primer extension reactions were conducted with the RT enzymes described except (A) 40-mer DNA template encoding the same sequence as the RNA template used in Figure 1 and (B) 48-mer DNA template encoding conserved HIV-1 PBS under the same reaction condition described in Figure 1. (C) Scheme explaining potential mechanistic ties between K m values of RT enzymes from lentiviruses encoding or non-encoding Vpx and cellular dNTP pools modulated by SAMHD1 and Vpx in macrophages.