Non-rooted molecular phylogenetic tree of flavonoid glycosyltransferases.The phylogenetic tree was constructed as described in Experimental Procedures. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches. The alignment used for this analysis is provided in Appendix S1. The scale bar = 0.1 amino acid substitutions per site. Flavonoid 2″-O-glycosyltransferases are shown in bold and UGT79B6 is shown in red. The Genbank accession numbers for the sequences are shown in parentheses: At3RhaT (NM_102790, At1g30530); At3GlcT (NM_121711, At5g17050); At3AraT (NM_121709, At5g17030); Vv3GlcT (AF000371); Ph3GalT (AF316552); AcF3GalT (GU079683); Ph3GlcT (AB027454); Pf3GlcT (AB002818); Hv3GlcT (X15694); Zm3GlcT (X13501); At5GlcT (NM_117485, At4g14090); Ph5GlcT (AB027455); Pf5GlcT (AB013596); Vh5GlcT (AB013598); At7GlcT (NM_129234, At2g36790); At7RhaT (NM_100480, At1g06000); DbB5GlcT (Y18871); NtIS5a (AF346431); Gt3′GlcT (AB076697); Sb7GlcT (AB031274); MtUGT72L1 (EU434684); UGT707B1, CsaF3G2″GlcT (HE793682); OsCGT (FM179712); Cm1,2RhaT, CmF7G2″RhaT (AY048882); BpA3G2″GlcAT (AB190262); CaUGT3, F3G6″GlcT (AB443870); Cs1,6RhaT, CsiF7G6″RhaT (DQ119035); F3GGT1, AcA3Ga2″XylT (FG404013); AtA3G2″XylT, UGT79B1 (NM_124785, At5g54060); AtF3G2″GlcT, UGT79B6 (NM_124780, At5g54010); IpA3G2″GlcT (AB192315); PhA3G6″RhaT, UGT79G16 (Z25802). A3G, anthocyanin 3-O-glucoside; A3Ga, anthocyanin 3-O-galactoide; F3G, flavonol 3-O-glucoside; F7G, flavonoid 7-O-glucoside; 3AraT, 3-O-arabinosyltransferase; 3GlcT, 3-O-glucosyltransferase; 3′GlcT, 3′-O-glucosyltransferase; 3GalT, 3-O-galactosyltransferase; 3RhaT, 3-O-rhamnosyltransferase; 5GlcT, 5-O-glucosyltransferase; 7GlcT, 7-O-glucosyltransferase; 7RhaT, 7-O-rhamnosyltransferase; 2″GlcT, 2″-O-glucosyltransferase; 2″RhaT, 2″-O-rhamnosyltransferase; 2″XylT, 2″-O-xylosyltransferase; 6″RhaT, 6″-O-rhamnosyltransferase; CGT, C-glucosyltransferase; NtIS5a, salicylate-induced glucosyltransferase. Abbreviations for species: Ac, Actinidia chinensis; At, Arabidopsis thaliana; Bp, Bellis perennis; Cm, Citrus maxima; Csa, Crocus sativus; Csi, Citrus sinensis; Db, Dorotheanthus bellidiformis; Gt, Gentiana triflora; Hv, Hordeum vulgare; Ip, Ipomoea purpurea; Nt, Nicotiana tabacum; Os, Oryza sativa; Pf, Perilla frutescens; Ph, Petunia hybrida; Sb, Scutellaria baicalensis; Vh, Verbena hybrida; Vv, Vitis vinifera; Zm, Zea mays.