Table 1.
Effect of MK-1775 on panobinostat sensitivities in pancreatic cancer cell lines.
| Cell line | IC50 of MK-1775 (μM) | IC50 of panobinostat (nM) in the absence or presence of MK-1775 (μM)
|
p Value# | |||
|---|---|---|---|---|---|---|
| 0 | 0.13 | 0.25 | 0.5 | |||
| ASPC-1 | 13.2 ± 1.1* | 94.6 ± 9.2 | 58.5 ± 6.3 (0.63) |
56.7 ± 4.8 (0.62) |
53.3 ± 3.7 (0.6) |
<0.05 |
| BxPC-3 | 0.8 ± 0.03 | 37.8 ± 2.2 | 16.2 ± 0.8 (0.59) |
13.2 ± 1.1 (0.66) |
11. 9 ± 1.0 (0.95) |
<0.001 |
| CFPAC-1 | 3.3 ± 0.2 | 407.6 ± 14.0 | 263.4 ± 19.9 (0.68) |
246.1 ± 7.6 (0.68) |
224.5 ± 5.0 (0.7) |
<0.005 |
| HPAC | 0.5 ± 0.01 | 81.7 ± 7.8 | 50.2 ± 1.6 (0.88) |
28.4 ± 1. 6 (0.88) |
ND | <0.05 |
| MIAPaCa-2 | 0.5 ± 0.05 | 36.0 ± 2.7 | 21.2 ± 1.5 (0.82) |
16.1 ± 1. 5 (0.91) |
ND | <0.05 |
| PANC-1 | 10.6 ± 1.1* | 569.6 ± 13.4* | 397.7 ± 23.0 (0.71) |
378.6 ± 24.3 (0.69) |
325.4 ± 21.6 (0.62) |
<0.005 |
Note: MK-1775 and panobinostat IC50s are presented as means ± standard errors from at least three independent experiments. Numbers in parentheses represent the combination index values.
ND – not determined.
*
indicates estimated IC50 calculated by using GraphPad Prism 5.0 software.
#
indicates that p values for each pair were determined using GraphPad Prism 5.0 and only the largest p value is shown for each cell line.