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Thermally denatured proteins do not generate C5a. The level of C5a measured by ELISA in the same serum samples analyzed in Figure 4. Panel A: serum incubated for 15 minutes at 37°C. Panel B: serum incubated for 60 minutes at 37°C. The α1PI blot showing iC3b:α1PI complex formation is displayed for comparison below the X-axis.
