Table 1.
Delta-9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), and 11-nor-9-carboxy-THC (THCCOOH) concentration changes from baseline for oral fluid specimens collected with the StatSure and Oral-Eze devices after being stored at room temperature (RT) for 1 week (Oral-Eze only), 4°C for 1 and 4 weeks, and –20°C for 4 and 24 weeks (both devices).
| Analyte | Baseline concentration range 1 | Storage condition | # Samples with analytes >LOQ2 | # Stable samples (%stable) | %Baseline, range |
|---|---|---|---|---|---|
| A. StatSure collection device | |||||
| THC | 6.8 – 509 μg/L | 1 week, 4°C | 16 | 16 (100) | 85.6–111 |
| 4 weeks, 4°C | 16 | 16 (100) | 82.3–111 | ||
| 4 weeks, –20°C | 16 | 16 (100) | 89.7–117 | ||
| 24 weeks, –20°C | 16 | 16 (100) | 84.6–110 | ||
| CBD | < LOQ –15.3 μg/L | 1 week, 4°C | 11 | 11 (100) | 94.3–109 |
| 4 weeks, 4°C | 10 | 10 (100) | 89.4–108 | ||
| 4 weeks, –20°C | 9 | 9 (100) | 88.4–108 | ||
| 24 weeks, –20°C | 9 | 9 (100) | 89.1–108 | ||
| CBN | 0.6 – 35.8 μg/L | 1 week, 4°C | 16 | 15 (94) | 80.4–167 |
| 4 weeks, 4°C | 15 | 13 (87) | 87.2–166 | ||
| 4 weeks, –20°C | 15 | 12 (80) | 91.9–1653 | ||
| 24 weeks, –20°C | 15 | 13 (87) | 90.3–183 | ||
| THCCOOH | < LOQ –150 ng/L | 1 week, 4°C | 12 | 12 (100) | 80.4–115 |
| 4 weeks, 4°C | 13 | 11 (85) | 68.4–142 | ||
| 4 weeks, –20°C | 13 | 11 (85) | 66.7–148 | ||
| 24 weeks, –20°C | 13 | 13 (100) | 88.3–118 | ||
| B. Oral-Eze collection device | |||||
| THC | 6 – 376 μg/L | 1 week, RT | 16 | 15 (94) | 70.7–108 |
| 1 week, 4°C | 16 | 16 (100) | 80.0–119 | ||
| 4 weeks, 4°C | 16 | 16 (100) | 80.1–119 | ||
| 4 weeks, –20°C | 16 | 15 (94) | 46.4–113 | ||
| 24 weeks, –20°C | 16 | 13 (81) | 60.5–99.8 | ||
| CBD | < LOQ – 15.1 μg/L | 1 week, RT | 8 | 7 (88) | 75.1–99.4 |
| 1 week, 4°C | 9 | 7 (78) | 66.1–111 | ||
| 4 weeks, 4°C | 9 | 8 (89) | 80.0–102 3 | ||
| 4 weeks, –20°C | 9 | 7 (78) | 40.8–99.8 | ||
| 24 weeks, –20°C | 9 | 5 (56) | 31.9–98.7 | ||
| CBN | <LOQ – 16.8 μg/L | 1 week, RT | 15 | 8 (53) | 70.2–155 |
| 1 week, 4°C | 15 | 9 (60) | 57.3–163 | ||
| 4 weeks, 4°C | 14 | 11 (79) | 20.1–147 | ||
| 4 weeks, –20°C | 13 | 9 (69) | 66.4–146 | ||
| 24 weeks, –20°C | 14 | 12 (86) | 83.2–129 | ||
| THCCOOH | < LOQ – 327 ng/L | 1 week, RT | 14 | 13 (93) | 70.1–120 |
| 1 week, 4°C | 13 | 13 (100) | 81.4–119 | ||
| 4 weeks, 4°C | 13 | 13 (100) | 82.4–118 | ||
| 4 weeks, –20°C | 13 | 12 (92) | 60.8–104 | ||
| 24 weeks, –20°C | 14 | 11 (79) | 75.1–139 | ||
1
No significant differences (p>0.05, Mann-Whitney test for median comparison) were observed between baseline concentrations after StatSure or Oral-Eze OF collections
2
LOQ: Limit of Quantification
3
1 CBD and 1 CBN sample concentration were quantified at >20% of LOQ at baseline but <LOQ after storage. No %baseline was calculated, but the sample was considered unstable.