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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mol Immunol. 2014 Nov 15;64(1):9–17. doi: 10.1016/j.molimm.2014.10.017

Figure 7. Absence of Factor D does not impair hepatocyte proliferation following CCl4-induced toxic injury.

Figure 7

WT and FD−/− mice were provided with a single injection of CCl4. A. Immunoreactive PCNA (proliferating cell nuclear antigen) protein in liver lysates was assessed by Western blot analysis 24, 48 and 72 hr after injection. Quantification of band intensity measured by densitometry after normalization to HSC70 showed no difference between genotypes (data not shown). B. Ki67 was visualized by immunohistochemistry in frozen liver sections 72 hr following injury. Total number of fluorescent pixels per 10× field was determined using Image Pro software. Images are representative of n = 4–6 mice per group. Bars are means ± SEM. Values with different alphabetical superscripts are significantly different from each other, p<.05.