Fig. 2.

dpres promoter region drives expression in chordotonal organs. a RT-PCR amplifies dpres mRNA from embryos, larvae, and adults. Without reverse transcriptase (RT) the longer genomic DNA contamination amplifies, but with RT, the shorter spliced RNA product is distinguished. b A 6.1 kb fragment of the dpres regulatory region (shown in Fig. 1) connected to Gal4 drives expression of GFP in the peripheral nervous system of embryos. Expression is seen in the neurons of the embryonic lateral chordotonal organs (lch5′s) (arrow and inset). c–e dpres-Gal4 also drives strong expression in JO neurons (c) in the second antennal segment (green: GFP, magenta: rhodamine phalloidin) and in the femoral chordotonal organ of the leg (d arrow) and the sensory organs of the anterior wing margin (e). Scale bars represent 40 μm in (b), 10 μm in (c), and 100 μm in (d) and (e)