Table 2.
Proportion of bacteria remaining in boar sperm samples after processing by single-layer (SLC) centrifugation through Androcoll-P (n = 10).
| Boar | Bacterial Load at 0 h after SLC (%) | Bacterial Load at 24 h after SLC (%) |
|---|---|---|
| 1 | 0 | 0 |
| 2 | 200 * | 0 |
| 3 | 0 | 0 |
| 4 | 0 | 0 |
| 5 | 0 | 0 |
| 6 | 0 | 0 |
| 7 | 5 | 2 |
| 8 | 5 | 6 |
| 9 | 83 * | 12 |
| 10 | 27 | 30 |
| total | <1 | <1 |
Note: There was possible re-contamination of sperm pellet after SLC, because the aliquot taken from the same SLC sample after 24 h had a much lower or no bacterial load. In addition, the contaminating bacteria were isolated in pure culture, in contrast to the original sample. Summarized from [29].