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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Immunol. 2014 Dec 10;194(2):522–530. doi: 10.4049/jimmunol.1400626

Figure 5. Early T cell arrest is antigen dependent.

Figure 5

BDC-2.5 T cells were fluorescently labeled and transferred into WT NOD or NOD.C6 recipient mice 48 hours prior to imaging to determine infiltration state. BDC-6.9 T cells and BDC-2.5 T cells were co-transferred 24 hours prior to imaging to determine T cell motility. The antigen for BDC-6.9 T cells is absent in the NOD.C6 recipients. Data represent 25 wild type islets from 4 mice in 4 experiments and 25 NOD.C6 islets from 5 mice in 5 experiments. Each point represents the average T cell motility within 1 islet. **= P<0.01, ***= P<0.001 by two-tailed Student’s t test. A) In WT NOD islets where the antigen was present for BDC-2.5 and BDC-6.9 T cells, both types of T cells increase motility at a similar rate as islet infiltration increases. B) In NOD.C6 islets, where the antigen is present for BDC-2.5 T cells, but absent for BDC-6.9 T cells, the BDC-6.9 T cells move faster in the absence of their antigen. C) The ratio of average BDC-6.9 T cell motility to the average BDC-2.5 T cell motility within the same islet. Infiltration states: very mild (0–5%), mild (5–30%), and advanced (30–60%). D) Comparison of the arrest coefficient of all BDC-2.5 and BDC-6.9 T cells within islets with mild infiltration from NOD.C6 mice. BDC-6.9 T cells (No Ag) have reduced arrest.