Fig. 3.

Immunofluorescence image of Cx34.7/Cx35 puncta in horizontal sections of the caudal portion of goldfish hindbrain at a rostro-caudal level of brainstem-spinal cord transition. Shown with and without blue fluorescence Nissl counterstaining. (A) Low magnification bilateral overview (dotted line indicates midline), showing collections of neurons on each side of the midline decorated with labeling for Cx34.7/Cx35 puncta around their somata (arrows) and along their laterally directed dendrites (arrowheads). A moderate density of labeling is seen distributed in more lateral regions of neuropil. (B-D) Regions indicated by single and double asterisks in A are shown in B and C, respectively, but taken by confocal scanning from different sections, and the boxed area in A is shown at higher magnification in D. In all regions, images show punctate appearance of immunolabeling, with no evidence of intracellular labeling. (E-G) Images of brainstem neurons taken for counts of Cx34.7/Cx35-puncta associated with neuronal somata (F,G arrows) and their initial dendrites. Images in F and G are magnifications of the boxed areas in E. Calibration bars are labeled individually on each image.