Fig. 4.

Immunofluorescence image revealing abundant Cx34.7/Cx35 puncta in selected regions of goldfish forebrain, shown in horizontal sections with blue fluorescence Nissl counterstaining. (A) Dorsal telencephalon, with dense immunolabeling scattered throughout and with greater abundance localized to patches of neuropil (arrows) or along dendrites directed towards the cortical surface (arrowheads). (B,C) Optic tectum and vagal lobe, both organized as laminar structures, with moderate immunolabeling localized to superficial, middle and deep lamina in the tectum (B, arrows) and dense dispersed labeling distributed also in three lamina of the vagal lobe (C, arrows). (D) Cerebellum, showing a high density of immunofluorescent puncta in the granule cell layer, with labeling clustered in relatively uniform size patches (arrows). (E,F) Torus semicircularis; labeling is sparse in most regions, except for puncta heavily concentrated on the somata and initial dendrites of a group of neurons arranged in an arc (E, arrows) spanning the length of this structure. As elsewhere, only higher magnification reveals the punctate nature of this labeling (F, arrows). Calibration bars are labeled individually on each image.