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Activation of GLAST was expressed as percent fluorescence intensity versus mean fluorescence intensity of control. Treatment of animals with bortezomib (n=5) significantly decreased expression of GLAST in the spinal dorsal horn versus saline-treated controls (n=6). This was partially prevented by co-treatment with minocycline (n=5), but decrease of GLAST was still significant in these animals. Minocyline alone (n=5) did not affect GLAST activity.
