Three-to-four week-old C57BL/6J WT (7 dpi, n = 6; 14 dpi, n = 6) and Rag1−/− (7 dpi, n = 3; 14 dpi, n = 6) mice were inoculated with 103 pfu by injection into the left rear footpad. At 7 and 14 dpi, mice were sacrificed, perfused by intracardial injection with 1x PBS, and total RNA was isolated from the (A) right quadriceps muscle and (B) right ankle/foot. RRV genomes were quantified by absolute RT-qPCR as described in the Materials and Methods. Horizontal bars indicate the mean. *** P < 0.001, as determined by one-way ANOVA followed by Tukey's multiple comparison test.