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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Immunol. 2014 Dec 8;194(2):678–689. doi: 10.4049/jimmunol.1401833

Figure 3. Virus-specific CD8+ T cells expand in RRV-LCMV-infected mice.

Figure 3

Three-to-four week-old C57BL/6J mice were mock-inoculated (n = 3) or inoculated with 103 PFU of WT RRV (n = 4) or RRV-LCMV (5 dpi, n = 6; 7 dpi n = 9; 10 dpi, n = 9; 14 dpi, n = 6). At the indicated day post-inoculation, leukocytes were isolated from spleens, draining popliteal lymph nodes (pLN), and quadriceps muscles (following enzymatic digestion) for FACS analysis. Spleen cells from a mouse inoculated with LCMV-Armstrong i.p. and harvested on day 7 pi was used as a control for gp33 tetramer staining. (A) Representative flow plots from 10 dpi indicating the gating strategy to identify CD8+CD4−gp33+ T cells, after gating on lymphocytes and excluding doublets. (B) Frequency (top panel) and total number (bottom panel) of CD8+CD4− T cells and CD8+CD4−gp33+ T cells in the spleen, pLN, and muscle tissues. Data are combined from 2–3 independent experiments. Graphs represent the arithmetic mean ± SEM. ** P < 0.01, as determined by two-way, unpaired t-tests.