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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Immunol. 2014 Dec 8;194(2):678–689. doi: 10.4049/jimmunol.1401833

Figure 6. CD8 T cell depletion significantly increases RRV loads in muscle but not joint tissue at 14.

Figure 6

Three-to-four week-old C57BL/6J WT mice were inoculated with 103 PFU of RRV and treated with an anti-CD8α antibody (n = 4) or a control antibody (n = 4) on days 7 and 12 pi. At 14 dpi mice were sacrificed and perfused by intracardial injection with 1× PBS. Spleens were harvested for FACS analysis; the right quadriceps and right ankle/foot were dissected for RNA analysis. Spleen cells were stained for CD3, CD4, and CD8β to confirm efficient depletion. (A) Representative flow plots demonstrating depletion of CD8α+ T cells. (B) Frequency of CD3+CD8β+ T cells in spleens. Total RNA was isolated from the (C) right quadriceps muscle and (D) right ankle/foot, and RRV genome levels were quantified by absolute RT-qPCR. Horizontal bars indicate the mean. P-value was determined by a two-tailed, unpaired t-test. Data are combined from two independent experiments.