Figure 2.
IEF gels showing the presence of small amounts of TBG with normal isoforms in hemizygous males. A, Note the presence of faint TBG isoform bands in serum of the hemizygotes of families 2 and 1 (subjects II-2 and II-3), compared to the total absence of TBG bands in the subject with TBG-CD. The excess labeled T4 not bound to TBG in the affected males is associated with transthyretin (TTR) and albumin (Alb). B, Addition of unlabeled T4 cleared the bands corresponding to the TBG isoforms in the samples from affected individuals of families 2 and 1 (lanes 3 and 6 counting from the left) and indicated by an asterisk, corroborating that the faint bands do correspond to TBG. Het, heterozygous; Hem, hemizygous.