Figure 5.

Expression of eRNA in the mutation locus in HepG2 cells. Representative image of a 3% agarose gel showing the expression of eRNA in the mutation locus in HepG2 cells (abbreviated eTBG). RNA was collected from HepG2 cells, treated with DNAse, precipitated with ethanol, and reverse transcribed to cDNA. A total of 200 ng of cDNA were used for the eTBG and 80ng for the RP2 (housekeeping gene) PCR. Plus sign denotes the presence and minus sign denotes the absence of reverse transcriptase during reverse transcription to control for gDNA contamination.