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. 2013 Nov 11;26(12):679–688. doi: 10.1002/jmr.2313

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© 2013 The Authors. Journal of Molecular Recognition published by John Wiley & Sons, Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Spot14/Mig12 heterocomplex prevents ACC2 polymerization. ACC2 polymerization with citrate or citrate + Spot14/Mig12 αβ was initially separated by gradient BN-PAGE and analyzed by Western blot using an anti-Flag antibody. (A) Incubation of ACC2 with citrate induced formation of high-molecular-weight ACC2 polymers, but addition of Spot14/Mig12 αβ diminished the generation of ACC2 polymers. (B) ACC2 and citrate were pre-incubated to produce nucleated ACC2 polymers, followed by additional incubation with Spot14/Mig12 αβ. Spot14/Mig12 heterocomplex addition could not further halt the polymerization process of nucleated ACC2 polymers. S/M αβ, Spot14/Mig12 heterocomplex.