Fold changes of classical aquaporin’s mRNA levels in D2 (n > 10), D9 (n > 10), and D30 (n = 8) cholangiocytes relative to that at D60 (n = 4) assayed by RT-qPCR. Panel configuration is as follows: A) AQP0, B) AQP1, C) AQP2, D) AQP4, and E) AQP5. The level of each AQP transcript was normalized to GAPDH. Statistically significant differences, relative to D60, are indicated in the following manner: * = p< 0.05, ** = p< 0.01, and *** = p< 0.001.