Table 1.1.
Examples of some important flow cytometric-measurable parameters and measurement techniques and/or fluorescent probes.
| Measurable variables | Measurement techniques and/or associated probe(s) | Examples |
|---|---|---|
| Cell count and size | Scatter dot plots | Nicotiana tabacum (9) |
| DNA, ploidy, cell cycle | DAPI, mithramycin, propidium iodide | Dioscorea (10) |
| Chlorophyll content | Autofluorescence | Nicotiana tabacum (11) |
| Cell viability | PI and fluorescein diacetate (FDA) | Brassica napus (12) |
| Cell-wall components | Specific antibodies, Calcofluor white | Brassica napus (13) |
| Apoptosis and related studies | Annexin-V | Nicotiana plumbaginifolia (14) |
| Membrane fluidity | Diphenylhexatriene (DPH) | Lupinus albus L. (15) |
| Membrane potential | Rhodamine 123 | Solanum tuberosum L. (16) |
| Intracellular Ca2+ | Indo-1, fura-2 | Barley aleurone (17) |
| Gene expression | GFP or other reporter genes | Arabidopsis (18) |
| Intracellular molecules | Specific dyes, antibodies tagged with fluorescence after cell fixation and permeabilization | Coptis japonica (19) |