Fig. 1.

Effect of MeJA elicitation on T. cuspidata P93AF cultures growth, taxane production and viability. (a) Biomass concentrations in MeJA-elicited cultures as compared to mock-elicited cultures. Reported values are the average of three biological replicates and error bars represent standard error of the mean (SEM). (b) Taxane levels as determined by UPLC in MeJA-elicited cultures. Note that mock-elicited cultures did not produce detectable levels of taxanes. Reported values are the average of three biological replicates and error bars represent SEM. (c, d) Viability of mock-elicited and MeJA-elicited cultures on day 16 (Row 1), day 21 (Row 2), and day 24 (Row 3) of the culture period. Columns indicate brightfield (Column 1), FDA-stained (Column 2) and PI-stained (Column 3) images. Cultures were either mock-elicited or elicited with 150 µM MeJA on day 7 of the culture period