Table 1.

Chromatographic gradient conditions for the analysis of GCA, BCA, and free amino acids.

Time (min)	Eluent Aa	Eluent Bb
0	96	4
3	88	12
13	88	12
30	69	31
35	69	31
40	96	4

^a25 mM glacial acetic acid, 0.02% (w/v) sodium azide pH 6.0.

^bAcetonitrile.