Figure 1.

Gating strategy. Gating strategy and presentation of monocyte subsets. (a) Exclusion of debris and doublets. (b) Identification of monocytes based on the forward scatter (FSC) versus side scatter (SSC) plot and CD86 positivity. (c) Separation of monocytes in CD16 positive and CD16 negative monocytes and subsequent distinction between Mon2 and Mon3, based on CCR2 expression. The plot shows all monocyte subsets, with Mon1 as per definition in the CCR2⁺ gate. (d) Respective location of the monocyte subsets on CD14 versus CD16 plot.