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. 2014 Nov 15;9(22):1955–1958. doi: 10.4103/1673-5374.145371

Figure 1.

Figure 1

Screening of a phage peptide library on neuron as the target.

After binding of some phages of the library to neurons, multiple steps of stringent washing result in the removal of weakly-bound or unbound phages. Phages with tight binding to neurons are eluted and amplified for the next round. Several rounds of biopanning ultimately lead to the isolation of phages that show specific binding to neurons.