Figure 1.

Effects of different concentrations of propofol and remifentanil on neural stem/ progenitor cell morphology (immunofluorescence staining, × 200).

The morphology of cells treated with low concentrations of propofol and/or remifentanil was not notably different from that of control cells. However, after treatment with high concentrations, differentiation was suppressed. Green: Astrocyte-specific glial fibrillary acidic protein (GFAP; FITC staining); red: neuron-specific β-tubulin (TRITC staining); blue: nuclei (DAPI staining). P₁: 1.0 μg/mL propofol; P₂: 2.5 μg/mL propofol; P₃: 5.0 μg/mL propofol; R₁: 5 ng/mL remifentanil; R₂: 10 ng/mL remifentanil; R₃: 20 ng/mL remifentanil; R₁ + P₁: 5 ng/mL remifentanil + 1.0 μg/mL propofol; R₂ + P₂: 10 ng/mL remifentanil + 2.5 μg/mL propofol; R₃ + P₃: 20 ng/mL remifentanil + 5.0 μg/mL propofol; FITC: fluorescein isothiocyanate; DAPI: 4′,6-diamidino-2-phenylindole.