Table 1. Macrophage depletion by clodronate.
|
Sgca-/- |
C57BL6 |
|||
|---|---|---|---|---|
| % CD11b+ F4/80+ | % CD11b+ Gr1+ | % CD11b+ F4/80+ | % CD11b+ Gr1+ | |
| PBS | 7.1±1.1 | 3.2±0.5 | 7.3±1.0 | 3.3±0.7 |
| Lipos.ctl | 7.8±0.9 | 3.7±0.7 | 7.5±0.7 | 3.8±0.5 |
| Lipos. clodronate | 2.6±0.7 | 3.0±0.6 | 1.8±0.9 | 3.1±0.7 |
Abbreviations: FACS, fluorescence-activated cell sorter; lipos.ctl, control liposomes lipos.Clodronate, liposomal clodronate; PBS, phosphate-buffered saline.
Each mouse was injected i.m. with 5 × 109 vg of rAAV2/1_CMV_SGCA-HY vector, and then received either PBS or lipos.clodronate or lipos.ctl as described in the Materials and methods section. Fourteen days after administration of the vector and 2 days after the last injection of liposomes, live spleen cells were analyzed by FACS and results show the mean percentages (plus/minus s.d.) of CD11b+ F4/80+ macrophages or of CD11b+ Gr1+ granulocytes of three separate experiments. Bold numbers emphasize the depletion of macrophages by clodronate-containing liposomes both in Scga-/- and in C57BL/6 mice.