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. 2014 Sep 19;33(21):2492–2506. doi: 10.15252/embj.201488208

Figure 1. SPP-Derlin1-TRC8 complex.

Figure 1

  1. Immunoprecipitation (IP) of CHAPS-solubilized HA-tagged SPP (SPP-HA) and Western blot (WB) analysis with indicated antibodies. Filled triangle, monomer of SPP-HA and endogenous (end.) SPP; open triangle, SDS-stable SPP dimer; asterisks, cross-reacting immunoglobulin chains.
  2. IP/WB analysis of endogenous SPP as in (A).
  3. IP/WB analysis of endogenous Derlin1 as in (A).
  4. BN-PAGE and WB analysis of immunoisolated digitonin-solubilized SPP-HA resolves a 200-kDa (SPP-C200) from a 500-kDa (SPP-C500) complex.

Source data are available online for this figure.