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. 2014 Sep 19;33(21):2492–2506. doi: 10.15252/embj.201488208

Figure 8. XBP1u tail targets a type II membrane protein for SPP-, TRC8-, and Derlin1-dependent degradation.

Figure 8

  1. Outline of domain fusions between CD74 (indicated in red) and XBP1u.
  2. CD74-XBP1u chimera was co-transfected either with empty vector (mock), SPPD265A, TRC8ΔR or Derlin1G180V as indicated and subjected to 35S-pulse-label chase analysis (means ± SEM, n = 3).
  3. Pulse-chase analysis of XBP1u-CD74 turnover as in (B).
  4. Knockdown of Derlin1 by siRNA reduces XBP1u degradation (means ± SEM, n = 3). CHX, cycloheximide chase; nt, non-targeting control siRNA.

Source data are available online for this figure.