Table 1.
Crystallization, X-ray data collection and refinement statistics for structures of wild-type elF5B(517–858)
| elF5B(517–858)·GTP·Na+ | elF5B(517–858)·GTPγS·Na+ | elF5B(517–858)·GTPγS·K+ | |
|---|---|---|---|
| Crystallization | |||
| Condition | 100 mM HEPES (pH 7.25), 12% PEG 4000, 100 mM NaOAc | 100 mM HEPES (pH 7), 13% PEG 4000, 125 mM NaOAc | 11% PEG 8000, 6% glycerol, 50 mM KCI |
| Temperature (°C) | 20 | 20 | 20 |
| Data collection | |||
| Space group | P21 | P21 | P41212 |
| Unit cell | a = 55.6 Å | a = 55.6 Å | a = 116.1 Å |
| b = 116.5 Å | b = 115.9 Å | b = 116.1 Å | |
| c = 66.2 Å | c = 66.1 Å | c = 120.3 Å | |
| α = 90° | α = 90° | α = 90° | |
| β = 101.1° | β = 101 4° | β = 90° | |
| γ = 90° | γ = 90° | γ = 90° | |
| Molecules/asym. unit | 2 | 2 | 2 |
| Resolution (Å) | 1.55 (1.64–1.55) | 1.53 (1.63–1.53) | 2.28 (2.42–2.28) |
| Observed reflections | 405,616 (64,352) | 510,862 (86,738) | 307,464 (49,685) |
| Unique reflections | 118,431 (18,849) | 121,606 (20,942) | 38,004 (6,021) |
| Completeness (%) | 98.8 (97.7) | 98.5 (98.4) | 99.9 (99.7) |
| <I>/σ | 15.5 (2.3) | 18.8 (2.8) | 19.3 (3.8) |
| Rsym (%) | 4.6 (50.5) | 3.7 (49.6) | 6.3 (54.2) |
| CC (1/2) (%) | 99.9 (75.1) | 99.9 (82.2) | 99.9 (99.1) |
| Refinement | |||
| Rwork (%) | 15.7 | 16.3 | 20.0 |
| Rfree (%) | 18.4 | 18.5 | 23.9 |
| Rmsd from standard stereochemistry | |||
| Bond length (Å) | 0.019 | 0.02 | 0.005 |
| Bond angles (°) | 1.8 | 1.9 | 1.0 |
| Ramachandran plot statistics | |||
| Most favored (%) | 96.9 | 98.1 | 98.5 |
| Allowed regions (%) | 1.1 | 1.9 | 1.5 |
| Disallowed regions (%) | 0 | 0 | 0 |
Values in parentheses refer to the highest resolution shell.
Rwork and Rfree factors are calculated using the formula 
, where f(obs)hkl and f(calc)hkl are observed and measured structure factors, respectively. Rwork and Rfree differ in the set of reflections they are calculated from: Rfree is calculated for the test set, whereas Rwork is calculated for the working set.