Table 2.
Changes in heat capacity and solvent accessible surface area for cteIF5B(517–858) binding to GTP, GTPγS, GDPNP and GDP
| Ligand | ΔCp [cal mol−1 K−1] | ΔASAmin [Å2] (with Δcmin = 0.45) | ΔASAmax [Å2] (with Δcmax = 0.24) |
|---|---|---|---|
| GTP | −553 ± 11 | 1,229 | 2,304 |
| GTPγS | −539 ± 30 | 1,198 | 2,254 |
| GDPNP | −197 ± 26 | 433 | 820 |
| GDP | −228 ± 10 | 507 | 950 |
ΔCp, heat capacity change; obtained from ΔH/dT.
Δcmin and Δcmax area coefficients in cal K−1 (mol Å2)−1 for calculation of ΔASA.
ΔASAmin and ΔASAmax, changes in solvent accessible surface areas assuming that all changes were conferred by either apolar or 70% apolar and 30% polar surfaces, respectively.
Obtained from (Kuhle & Ficner, 2014).
Obtained from the slope of the linear fit to ΔH measured at different temperatures between 10 and 30°C.