Table 3.

Intrinsic GTPase activities of wt and mutant ctelF5B(517–858) and ecEF-Tu

Protein	Construct	KCl (mM)	k (min−1)	Fold reduction compared to wild-type at 200 mM KCl
ctelF5B	wt	200	0.023 ± 0.0015
	wt	0	0.0021 ± 0.001	11
	D533A	200	0.0018 ± 0.0005	13
	D533A	0	0.0016 ± 0.0008	14
	D533N	200	0.017 ± 0.002	1.4
	D533R	200	0.0028 ± 0.0007	8
ecEF-Tu	wt	200	0.026 ± 0.0005
	wt	0	0.003 ± 0.0002	9
	D21A	200	0.0035 ± 0.0008	7
	D21A	0	0.0041 ± 0.0001	6

Measurements were performed two to three times.

k, rate of GTP hydrolysis under the used experimental conditions.

Measured under multiple turnover conditions at 35°C.

Measured under single turnover conditions at 30°C.