Table 1.
Oligonucleotides used in this study.
| Name | Sequence | Purpose |
|---|---|---|
| P6 | 5′-GAG TGC ACC ATA TGG ACA TAT TGT C-3′ | Gene targeting |
| T7 | 5′-CGA TAA GCC AGG TTA ACC TGC ATT A-3′ | Gene targeting |
| N1 | 5′-TGC GAG GCC AGA GGC CAC TTG TGT AGC-3′ | Gene targeting |
| N2 | 5′-TTC CTC GTG CTT TAC GGT ATC G-3′ | Gene targeting |
| loxP | 5′-TGA GAA ACA TGT ATG TGG GGC ATG-3′ | Gene targeting |
| LOX2 | 5′-GTA ACC ATG ATT AGT CTT TTA GCT TTA TG-3′ | Gene targeting, genotyping |
| SDL2 | 5′-GTT CTG AGA ATC CAA AGC ACA AC-3′ | Gene targeting, genotyping |
| NDEL1 | 5′-GTT GTG CTT TGG ATT CTC AGA AC-3′ | Gene targeting |
| NDEL2 | 5′-CTT CTA CCC TTC TAT TTC AGG AAG-3′ | Gene targeting |
| A1 | 5′-CAC AAA TAC CTG CAA CAC CAG TGG-3′ | Gene targeting |
| A2 | 5′-ACG CCA GTG TCA TGT TGT TG-3′ | Gene targeting |
| A3 | 5′-GGA AAC AGA TAA CAT GCA TCA TAA ACA-3′ | Gene targeting |
| AT1 | 5′-AAAT CGT AGG CTA GTA CTC CAC CC-3′ | Gene targeting |
| AT2 | 5′-AGC TCA GGA AAA GCT TCC TG-3′ | Gene targeting |
| F3 | 5′-GCA TAA GCT TGG ATC CGT TCT TCG GAC-3′ | Gene targeting |
| F7 | 5′-GGA ACT TCG CTA GAC TAG TAC GCG TG-3′ | Gene targeting |
| LUNI | 5′-GCA TCG CCT TCT ATC GCC TTC TTG-3′ | Gene targeting |
| PB1.1 | 5′-TTT TGT GGC TAA GCA CAGT CCC-3′ | Southern blot probe |
| PB1.2 | 5′-CAT ACT GCA GCT CTG CTC AGA TCC-3′ | Southern blot probe |
| PB2.1 | 5′-TGA CTG CCC TCA ACA GTT CAT TTG-3′ | Southern blot probe |
| PB2.2 | 5′-ATT CAG GAT CTG CTA CCA CCA CTG-3′ | Southern blot probe |
| Cre320 sense | 5′-GAA CCT GAT GGA CAT GTT CA-3′ | Genotyping |
| Cre320 anti | 5′-AGT GCG TTC GAA CGC TAG AGC CTG T-3′ | Genotyping |
| NFAT5 sense | 5′-AAC ATT GGA CAG CCA AAA GG-3′ | qRT-PCR analysis |
| NFAT5 anti | 5′-GCA ACA CCA CTG GTT CAT TA-3′ | qRT-PCR analysis |
| AR sense | 5′-CTT AAA ATA TAA GCC TGC GGT GA-3′ | qRT-PCR analysis |
| AR anti | 5′-GCC TTT GCT GTG GCA GTA TT-3′ | qRT-PCR analysis |
| BGT-1 sense | 5′-GGC TCC TTT TGG TCA CAG AG-3′ | qRT-PCR analysis |
| BGT-1 anti | 5′-GCT GGA GGC GTA GTA GTC AAA-3′ | qRT-PCR analysis |
| HSP70 sense | 5′-TGA GTC CCA CAC TCT CAC CA-3′ | qRT-PCR analysis |
| HSP70 anti | 5′-CTG TGG GTG AAG CTG TTA AGG-3′ | qRT-PCR analysis |
| β-Actin sense | 5′-CTA AGG CCA ACC GTG AAA AG-3′ | qRT-PCR analysis |
| β-Actin anti | 5′-ACC AGA GGC ATA CAG GGA CA-3′ | qRT-PCR analysis |