Figure 2.
Organization and transcription of the CRISPR-array of C. jejuni PT14. The CRISPR array is located between moe2 (A911_07320) and cas2 (A911_07325). The plot shows log2 cDNA read counts from libraries constructed from TEX treated C. jejuni PT14 RNA mapped to the CRISPR array. The relative positions of direct repeats are indicated as black squares, the spacers as green diamonds (spacers 1–3) and the tracrRNA in gold. Transcription start sites are marked by black arrows where the greater transcription arising from spacer 2 indicated by the width of the arrow. The −10 nucleotide motifs with respect to the transcription start sites are indicated adjacent to the arrows for the spacer 2 and the tracrRNA, and those common to the direct repeats below the black squares. The position of spacer acquisition in the carrier state cultures is indicated below (spacer 4).