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. 2015 Jan 5;5:659. doi: 10.3389/fimmu.2014.00659

Figure 4.

Figure 4

Identification of the specific PKC isoform target to enhance apoptosis in HT1080 cells. (A) Relative mRNA expressions of 10 PKC isoforms in HT1080 unstimulated cells. (B) Effect of siRNA knock-down (KD) for PKCα, PKCι, PKCε, and PKCδ. HT1080 cells were incubated in the presence of each PKC isoform siRNA (50 μM) for 24 h. Relative mRNA expressions of four PKC isoforms are measured by qRT-PCR. (C) Cell viability assay (trypan blue) of HT1080 cells incubated in the presence of PKC isoforms siRNA (50 μM) for 3 h. Error bars indicate mean values ± SD. for n = 3 independent experiments.