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. 2014 Nov 7;8(1):65–80. doi: 10.1242/dmm.017145

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — CREM/ICER attenuated CRE-HIF-1α activation in diabetic SIECs. (A) qRT-PCR analysis of CREM/ICER1 mRNA in control and diabetic SIECs. Actin was used as loading control. (B) Representative images of immunofluorescent staining of CREM-1α (CREM) in control and diabetic SIECs. (C) Quantitative measurement of the fold change in the intensity of the CREM-1α fluorescence shown in B. (D) SIECs were incubated in the presence or absence of 100 μM of MB-cAMP for various time intervals, and then ICER mRNA levels were determined by using qRT-PCR. ‘C’, control; ‘D’, diabetic. Results are expressed as means±s.e.m. from three independent experiments. *Significantly different from corresponding control values at P≤0.05. **Significantly different from corresponding MB-cAMP-treated control values at P≤0.05.