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. 2014 Nov 7;8(1):93–104. doi: 10.1242/dmm.018622

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Effects of oestrogen (E2) supplementation on 52-week-old OVX mice. OVX and E2 pellet implantation were performed at an age of 41 weeks. E2 increased uterus weight and oestrogen serum levels, and reversed the osteopenic phenotype of non-supplemented OVX mice. The dotted lines indicate the values of aged-matched non-OVX mice. (A) Images of representative uteri. (B) Uterus weight. n=7–12. (C) Oestrogen serum levels determined using E2-ELISA. n=6. (D) Representative μCT sections of the distal femur and the lumbar spine (L6). (E–H) μCT evaluation of trabecular bone (Tb.) in the distal femur. n=8. (E) Trabecular bone volume/total volume, Tb. BV/TV; (F) trabecular number, Tb.N; (G) trabecular thickness, Tb.Th; (H) trabecular spacing, Tb.Sp; and (I) cortical thickness, C.Th. *P<0.05.