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. 2014 Feb 26;64(1):11–19. doi: 10.1136/gutjnl-2013-306171

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/

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Compartmentalisation and quantitative analysis of proliferation and mitosis. (A) Representation of a section of human oesophageal epithelium. (B) Whole mounts stained for the proliferation marker Ki67 (green) representative of interpapillary basal layer (IBL, i), base (ii), middle (iii) and tip (iv) of the papillae. (C–E) Quantitative analysis of proliferation (C) and mitosis (E) in the four-cell compartments expressed as percentage of Ki67 and PH3-positive cells, respectively. Data points represent the average proliferation rate in each individual whole mount or patient sample and the statistical analysis assesses the significance of the overall trend across the four regions. (D). Whole mounts stained for the mitotic marker PH3 (red) representative of interpapillary epithelium (i), base (ii), middle (iii) and tip (iv) of the papillae. Blue colour identifies DAPI nuclear staining.