Figure 3.

Confirmation of the epithelial lineage of cells expressing putative stem cell markers and exclusion of other possible cell origins. (A) Confocal section of the tip of a papilla following labelling for β1-integrin (green) and PanCK (red). (B) 3D projection of a confocal z-stack of the tip of a papilla following co-staining for β1-integrin (i, green) and the endothelial marker CD31 (i and ii, red); the white arrows in both pictures indicate the cells that express β1-integrin but not CD31. (C) Confocal sections of oesophageal whole mounts acquired following staining for the lymphocyte marker CD45 (i, green), β1-integrin and the neural crest marker S-100 (ii, green and red, respectively), β1-integrin and the macrophage marker F4-80 (iii, green and red, respectively), β1-integrin and the Merkel cell marker chromogranin A (iv, green and red, respectively). DAPI was used as counterstain (blue).