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. 2014 Feb 26;64(1):11–19. doi: 10.1136/gutjnl-2013-306171

Figure 5.

Figure 5

Characterisation of the 3D in vitro cultures. Characterisation of 3D organotypic cultures from oesophageal cells sorted on the basis of EpCAM and CD34. (A) Representative images of 3D organotypic cultures grown stained with haematoxylin and eosin (H&E) from CD34−/EpCAM+ cells (i), CD34−/EpCAM− cells (ii) and CD34+/EpCAM− cells (×20 M, ×40, iii and iv, respectively). (B) Representative images of outgrowths stained for Ki67 (ii), MCM2 (ii), β1-integrin (iii) and p63 (iv). (A) Representative images of the same outgrowths in H&E (i), or stained for CK13 (ii), E-Cadherin (ii) and PanCK (iv).