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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Thromb Haemost. 2012 Nov 22;109(1):53–60. doi: 10.1160/TH12-05-0316

Figure 1. Effect of elevated FVIII levels on the formation of TAT complexes.

Figure 1

FVIII levels were elevated in C57Bl/6 FVIII−/− plasma through the addition of rhFVIII: 0% FVIII activity (■), 100% FVIII activity (▲), 200% FVIII activity (▼), and 400% FVIII activity (♦). Coagulation was stimulated through the addition of tissue factor and calcium chloride. The concentration of formed TAT complexes was quantified using TAT complex ELISA. The propagation rate, calculated from the slope between 5 minutes and 15 minutes of the TAT complex generation curve, was significantly increased in samples with 400% FVIII activity, when compared to samples with 100% FVIII activity (P<.05, Tukey’s post test). The error bars are representative of the standard error of the mean of 6 experiments.