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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Thromb Haemost. 2012 Nov 22;109(1):53–60. doi: 10.1160/TH12-05-0316

Figure 4. Time to vessel occlusion and platelet accumulation following endothelial damage in mice with extended elevation of circulating FVIII levels.

Figure 4

Injury of cremaster muscle arterioles (50–75 µm in diameter) was induced through the application of 10% ferric chloride soaked filter paper and injured arterioles observed for 40 minutes. FVIII levels were elevated in C57Bl/6 normal mice through hydrodynamic injection of the pSC11-ET-mFviii-plasmid, containing the murine B-domain deleted FVIII cDNA. (A) Time to vessel occlusion. Extended elevation of FVIII levels did not significantly decrease time to vessel occlusion. Each symbol represents one mouse. (B) Total fluorescence accumulation. Platelet accumulation was determined from the area under the fluorescence intensity curve. Total platelet accumulation was not significantly increased in mice with elevated FVIII levels. The error bars are representative of the standard error of the mean (N=4–6 mice).