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. Author manuscript; available in PMC: 2015 Jan 5.
Published in final edited form as: Vaccine. 2013 Nov 18;32(2):265–270. doi: 10.1016/j.vaccine.2013.11.013

Figure 2. Cytokine induction by TLR-3 (pI:C) and TLR-9 (ODN2216) agonists in neonatal and adult cotton rat spleen cells.

Figure 2

Neonatal (day 3 old) and adult (week 7 old) spleen cells were stimulated with different concentrations of pI:C, ODN 2216 or a combination of both (1:1 ratio). A. Supernatants were tested for the presence of type I interferon by bioassay. (A representative experiment of three is shown). B. IL-6 mRNA was quantified by PCR from cell pellets. C. IL-10 mRNA was quantified by PCR from cell pellets. The number of asterisks denotes the level of statistical significance (**p < 0.01).