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. 2015 Jan 5;5:7626. doi: 10.1038/srep07626

Figure 5. TGF-β1 inhibits NF-κB transcriptional activity by upregulating LBH expression in NP69 cells.

Figure 5

(a) qRT-PCR analysis showed that TGF-β1 induced LBH mRNA expression in NP69 cells (#P < 0.01 vs. control group). (b) A dual-reporter assay showed that LBH expression or TGF-β1 treatment downregulated NF-κB transcriptional activity in NP69 cells and that LBH expression and TGF-β1 demonstrated some synergistic effects on inhibiting NF-κB transcriptional activity (*P < 0.05, LBH(+) group vs. LBH(−) group; **P < 0.05, LBH(+)-TGFβ1 group vs. LBH(+) group). (c) NF-κB transcriptional activity was inhibited in NP69 cells after TGF-β1 treatment, but this inhibition effect was no longer significant after LBH knockdown (*P < 0.05, NC-siRNA-TGF-β1 vs. NC- siRNA-con group). (d) TGF-β1 at the 10 ng/mL and 12.5 ng/mL doses inhibited NP69 cell proliferation (*P < 0.05 vs. NP69 group). (e) TGF-β1 had no effect on NP69 cell proliferation after LBH knockdown.