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. 2014 Dec 5;53(51):8061–8076. doi: 10.1021/bi501216a

Figure 3.

Figure 3

Influence of the DNA substrate primer terminal sugar manifested in ionic current traces for captured Φ29 DNAP complexes. (a) DNA sequence of the hairpin primer–template substrates used in the study. Each substrate features a 14-base pair duplex region and a single-stranded template region of 35 nucleotides. The template strands contain a reporter group of five consecutive abasic (1′,2′-H) residues spanning positions +8 to +12 (indicated as red Xs in the sequence). (b) Ionic current traces for individual binary complexes formed between the D12A/D66A mutant of Φ29 DNAP with (i) DNA1-H_H, which bears a 2′-H, 3′-H primer terminus, (ii) DNA1-OH_H, which bears a 2′-OH, 3′-H primer terminus, (iii) DNA1-H_OH, which bears a 2′-H, 3′-OH (natural deoxyribose) primer terminus, or (iv) DNA1-OH_OH, which bears a 2′-OH, 3′-OH (natural ribose) primer terminus. Complexes were captured at 180 mV.