Figure 6.

Effects of a 2′-OH, 3′-OH primer terminated substrate on the rates of translocation and primer strand transfer between the polymerase and exonuclease sites. Plots of (a) log(r₁) vs voltage, (b) log(r₂) vs voltage, (c) log(r₃) vs voltage, and (d) log(r₄) vs voltage for complexes formed between DNA1-H_OH (magenta symbols) or DNA1-OH_OH (green symbols) and D12A/D66A (◆) or D12A/D66A/Y254V (▶) Φ29 DNAP. Rates were determined from dwell time samples extracted from ionic current traces and a three-state kinetic model⁴⁰ consisting of transitions r₁, r₂, r₃, and r₄ in the model diagram in Figure 1a. Errors bars indicate the standard error.